The Tenuta San Guido is a 7,500-acre estate located in the province of Livorno on the western coastal outskirts of Tuscany near the village of Bolgheri. Marchese Mario Incisa della Rocchetta acquired it through his marriage to Clarice della Gherardesca in 1940.

The legacy of Sassicaia began in 1944, when Mario Incisa acquired a number of Cabernet Sauvignon and Franc vine cuttings and planted them on a sloping hillside of the San Guido estate, called Castiglioncello after the 11th-century castle at the vineyard's upper edge. This tiny, 3.75-acre vineyard stood alone until 1965, when a second Cabernet vineyard was planted with cuttings from the Castiglioncello parcel , the gravelly, 30-acre plot would give the wine its name: Sassicaia, "the place of many stones".

With the radical changes in the D.O.C. system of regulations as of the 1994 vintage, Sassicaia's extraordinary reputation was acknowledged through the Italian government's granting the wine its own appellation.

Sassicaia is today considered to be the new plus ultra of Italy's great red wines for its consistent excellence and its intuitive spirit. Acclaimed by the wine world's most respected voices, Sassicaia remains the legacy of its creator, Marchese Mario Incisa della Rocchetta, and his son, Marchese Nicol˜ Incisa della Rocchetta.